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m1 cells wells  (InvivoGen)


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    InvivoGen m1 cells wells
    siRNA knockdown of VSIG4 attenuates the M2c phenotype, and 12A12c repolarizes M2c-macrophages toward an <t>M1-like</t> phenotype ( A ) siRNA knockdown of VSIG4 in M2c macrophages transfected with 25 nM VSIG4 siRNA LNPs on days 1 and 3 during the differentiation and polarization period. VSIG4 mRNA and protein levels were measured on day 8 by branched chain DNA analysis and flow cytometry, respectively ( n = 4 donors). Green represents M1 macrophages, black represents M2 macrophages, red represents M2 macrophages transfected with VSIG4 siRNA. ( B ) The fold change of M2c markers CD16, CD163, and CD206 as determined by flow cytometry in M2c macrophages treated with siRNA LNPs relative to those treated with luciferase siRNA. ( C ) Flow cytometry determined the fold change of the M2c marker CD163 based on the % CD163 + of CD11b + CD3 − CD45 + M2c macrophages treated with 12A12 for 24 h followed by 0.1 <t>ng/mL</t> <t>LPS</t> for 24 h. ( D ) The fold change based on pg/mL secreted proteins measured by a 25-plex Luminex assay from human monocyte-derived M2c macrophages treated with 12A12c or isotype control for 30 min at 37 °C followed by 0.1 ng/mL LPS for 24 h. Fold changes were calculated with respect to the human IgG4 isotype control antibody for each donor and each concentration. Black lines represent individual donors ( n = 5). Purple lines represent the mean fold change across all donors.
    M1 Cells Wells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m1 cells wells/product/InvivoGen
    Average 97 stars, based on 1164 article reviews
    m1 cells wells - by Bioz Stars, 2026-06
    97/100 stars

    Images

    1) Product Images from "Antibodies Targeting Human or Mouse VSIG4 Repolarize Tumor-Associated Macrophages Providing the Potential of Potent and Specific Clinical Anti-Tumor Response Induced across Multiple Cancer Types"

    Article Title: Antibodies Targeting Human or Mouse VSIG4 Repolarize Tumor-Associated Macrophages Providing the Potential of Potent and Specific Clinical Anti-Tumor Response Induced across Multiple Cancer Types

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25116160

    siRNA knockdown of VSIG4 attenuates the M2c phenotype, and 12A12c repolarizes M2c-macrophages toward an M1-like phenotype ( A ) siRNA knockdown of VSIG4 in M2c macrophages transfected with 25 nM VSIG4 siRNA LNPs on days 1 and 3 during the differentiation and polarization period. VSIG4 mRNA and protein levels were measured on day 8 by branched chain DNA analysis and flow cytometry, respectively ( n = 4 donors). Green represents M1 macrophages, black represents M2 macrophages, red represents M2 macrophages transfected with VSIG4 siRNA. ( B ) The fold change of M2c markers CD16, CD163, and CD206 as determined by flow cytometry in M2c macrophages treated with siRNA LNPs relative to those treated with luciferase siRNA. ( C ) Flow cytometry determined the fold change of the M2c marker CD163 based on the % CD163 + of CD11b + CD3 − CD45 + M2c macrophages treated with 12A12 for 24 h followed by 0.1 ng/mL LPS for 24 h. ( D ) The fold change based on pg/mL secreted proteins measured by a 25-plex Luminex assay from human monocyte-derived M2c macrophages treated with 12A12c or isotype control for 30 min at 37 °C followed by 0.1 ng/mL LPS for 24 h. Fold changes were calculated with respect to the human IgG4 isotype control antibody for each donor and each concentration. Black lines represent individual donors ( n = 5). Purple lines represent the mean fold change across all donors.
    Figure Legend Snippet: siRNA knockdown of VSIG4 attenuates the M2c phenotype, and 12A12c repolarizes M2c-macrophages toward an M1-like phenotype ( A ) siRNA knockdown of VSIG4 in M2c macrophages transfected with 25 nM VSIG4 siRNA LNPs on days 1 and 3 during the differentiation and polarization period. VSIG4 mRNA and protein levels were measured on day 8 by branched chain DNA analysis and flow cytometry, respectively ( n = 4 donors). Green represents M1 macrophages, black represents M2 macrophages, red represents M2 macrophages transfected with VSIG4 siRNA. ( B ) The fold change of M2c markers CD16, CD163, and CD206 as determined by flow cytometry in M2c macrophages treated with siRNA LNPs relative to those treated with luciferase siRNA. ( C ) Flow cytometry determined the fold change of the M2c marker CD163 based on the % CD163 + of CD11b + CD3 − CD45 + M2c macrophages treated with 12A12 for 24 h followed by 0.1 ng/mL LPS for 24 h. ( D ) The fold change based on pg/mL secreted proteins measured by a 25-plex Luminex assay from human monocyte-derived M2c macrophages treated with 12A12c or isotype control for 30 min at 37 °C followed by 0.1 ng/mL LPS for 24 h. Fold changes were calculated with respect to the human IgG4 isotype control antibody for each donor and each concentration. Black lines represent individual donors ( n = 5). Purple lines represent the mean fold change across all donors.

    Techniques Used: Knockdown, Transfection, Flow Cytometry, Luciferase, Marker, Luminex, Derivative Assay, Control, Concentration Assay



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    siRNA knockdown of VSIG4 attenuates the M2c phenotype, and 12A12c repolarizes M2c-macrophages toward an <t>M1-like</t> phenotype ( A ) siRNA knockdown of VSIG4 in M2c macrophages transfected with 25 nM VSIG4 siRNA LNPs on days 1 and 3 during the differentiation and polarization period. VSIG4 mRNA and protein levels were measured on day 8 by branched chain DNA analysis and flow cytometry, respectively ( n = 4 donors). Green represents M1 macrophages, black represents M2 macrophages, red represents M2 macrophages transfected with VSIG4 siRNA. ( B ) The fold change of M2c markers CD16, CD163, and CD206 as determined by flow cytometry in M2c macrophages treated with siRNA LNPs relative to those treated with luciferase siRNA. ( C ) Flow cytometry determined the fold change of the M2c marker CD163 based on the % CD163 + of CD11b + CD3 − CD45 + M2c macrophages treated with 12A12 for 24 h followed by 0.1 <t>ng/mL</t> <t>LPS</t> for 24 h. ( D ) The fold change based on pg/mL secreted proteins measured by a 25-plex Luminex assay from human monocyte-derived M2c macrophages treated with 12A12c or isotype control for 30 min at 37 °C followed by 0.1 ng/mL LPS for 24 h. Fold changes were calculated with respect to the human IgG4 isotype control antibody for each donor and each concentration. Black lines represent individual donors ( n = 5). Purple lines represent the mean fold change across all donors.
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    Image Search Results


    siRNA knockdown of VSIG4 attenuates the M2c phenotype, and 12A12c repolarizes M2c-macrophages toward an M1-like phenotype ( A ) siRNA knockdown of VSIG4 in M2c macrophages transfected with 25 nM VSIG4 siRNA LNPs on days 1 and 3 during the differentiation and polarization period. VSIG4 mRNA and protein levels were measured on day 8 by branched chain DNA analysis and flow cytometry, respectively ( n = 4 donors). Green represents M1 macrophages, black represents M2 macrophages, red represents M2 macrophages transfected with VSIG4 siRNA. ( B ) The fold change of M2c markers CD16, CD163, and CD206 as determined by flow cytometry in M2c macrophages treated with siRNA LNPs relative to those treated with luciferase siRNA. ( C ) Flow cytometry determined the fold change of the M2c marker CD163 based on the % CD163 + of CD11b + CD3 − CD45 + M2c macrophages treated with 12A12 for 24 h followed by 0.1 ng/mL LPS for 24 h. ( D ) The fold change based on pg/mL secreted proteins measured by a 25-plex Luminex assay from human monocyte-derived M2c macrophages treated with 12A12c or isotype control for 30 min at 37 °C followed by 0.1 ng/mL LPS for 24 h. Fold changes were calculated with respect to the human IgG4 isotype control antibody for each donor and each concentration. Black lines represent individual donors ( n = 5). Purple lines represent the mean fold change across all donors.

    Journal: International Journal of Molecular Sciences

    Article Title: Antibodies Targeting Human or Mouse VSIG4 Repolarize Tumor-Associated Macrophages Providing the Potential of Potent and Specific Clinical Anti-Tumor Response Induced across Multiple Cancer Types

    doi: 10.3390/ijms25116160

    Figure Lengend Snippet: siRNA knockdown of VSIG4 attenuates the M2c phenotype, and 12A12c repolarizes M2c-macrophages toward an M1-like phenotype ( A ) siRNA knockdown of VSIG4 in M2c macrophages transfected with 25 nM VSIG4 siRNA LNPs on days 1 and 3 during the differentiation and polarization period. VSIG4 mRNA and protein levels were measured on day 8 by branched chain DNA analysis and flow cytometry, respectively ( n = 4 donors). Green represents M1 macrophages, black represents M2 macrophages, red represents M2 macrophages transfected with VSIG4 siRNA. ( B ) The fold change of M2c markers CD16, CD163, and CD206 as determined by flow cytometry in M2c macrophages treated with siRNA LNPs relative to those treated with luciferase siRNA. ( C ) Flow cytometry determined the fold change of the M2c marker CD163 based on the % CD163 + of CD11b + CD3 − CD45 + M2c macrophages treated with 12A12 for 24 h followed by 0.1 ng/mL LPS for 24 h. ( D ) The fold change based on pg/mL secreted proteins measured by a 25-plex Luminex assay from human monocyte-derived M2c macrophages treated with 12A12c or isotype control for 30 min at 37 °C followed by 0.1 ng/mL LPS for 24 h. Fold changes were calculated with respect to the human IgG4 isotype control antibody for each donor and each concentration. Black lines represent individual donors ( n = 5). Purple lines represent the mean fold change across all donors.

    Article Snippet: On day 6 of the assay, differentiated M2 cells were polarized to M2c macrophages with 1 mL of fresh media containing 50 ng/mL M-CSF and 10 ng/mL of interleukin-10 (IL-10, Cat #574004, Biolegend, San Diego, CA, USA) while M1 cells wells were polarized with 100 ng/mL LPS (Cat #tlrl-eblps, Invivogen, San Diego, CA, USA) and 20 ng/mL IFNg (Cat #570204, Biolegend, San Diego, CA, USA).

    Techniques: Knockdown, Transfection, Flow Cytometry, Luciferase, Marker, Luminex, Derivative Assay, Control, Concentration Assay